Product Name :
5-Ethynyl-2′-deoxyuridine
Description:
5-Ethynyl-2′-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2′-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.
CAS:
61135-33-9
Molecular Weight:
252.22
Formula:
C11H12N2O5
Chemical Name:
5-ethynyl-1-[(2R, 4S, 5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1, 2, 3, 4-tetrahydropyrimidine-2, 4-dione
Smiles :
C#CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O
InChiKey:
CDEURGJCGCHYFH-DJLDLDEBSA-N
InChi :
InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1
Purity:
≥98% (or refer to the Certificate of Analysis)
Shipping Condition:
Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition :
Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.{{Temozolomide} medchemexpress|{Temozolomide} Apoptosis|{Temozolomide} Technical Information|{Temozolomide} Purity|{Temozolomide} custom synthesis|{Temozolomide} Autophagy}
Shelf Life:
≥360 days if stored properly.{{Octreotide} medchemexpress|{Octreotide} Somatostatin Receptor|{Octreotide} Purity & Documentation|{Octreotide} Purity|{Octreotide} custom synthesis|{Octreotide} Cancer}
Stock Solution Storage:
0 – 4 oC for 1 month or refer to the Certificate of Analysis.
Additional information:
5-Ethynyl-2′-deoxyuridine (EdU), a thymidine analogue, is incorporated into cellular DNA during DNA replication and the subsequent reaction of EdU with a fluorescent azide in a “Click” reaction. EdU staining is a fast, sensitive and reproducible method to study cell proliferation. 5-Ethynyl-2′-deoxyuridine is an alkyl chain-based PROTAC linker that can be used in the synthesis of PROTACs.|Product information|CAS Number: 61135-33-9|Molecular Weight: 252.22|Formula: C11H12N2O5|Synonym:|EdU|5-EdU|2′-Deoxy-5-ethynyluridine|ethynyldeoxyuridine|Chemical Name: 5-ethynyl-1-[(2R, 4S, 5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1, 2, 3, 4-tetrahydropyrimidine-2, 4-dione|Smiles: C#CC1=CN([C@H]2C[C@H](O)[C@@H](CO)O2)C(=O)NC1=O|InChiKey: CDEURGJCGCHYFH-DJLDLDEBSA-N|InChi: InChI=1S/C11H12N2O5/c1-2-6-4-13(11(17)12-10(6)16)9-3-7(15)8(5-14)18-9/h1,4,7-9,14-15H,3,5H2,(H,12,16,17)/t7-,8+,9+/m0/s1|Technical Data|Appearance: Solid Power|Purity: ≥98% (or refer to the Certificate of Analysis)|Solubility: DMSO : 50 mg/mL (198.24 mM; Need ultrasonic) H2O : 25 mg/mL (99.12 mM; Need ultrasonic) Ethanol : 5 mg/mL (19.82 mM; Need ultrasonic)|Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis|Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.|Shelf Life: ≥360 days if stored properly.|Stock Solution Storage: 0 – 4 oC for 1 month or refer to the Certificate of Analysis.|Drug Formulation: To be determined|HS Tariff Code: 382200|How to use|In Vitro:|5-Ethynyl-2′-deoxyuridine (EdU) staining is a fast, sensitive and reproducible method to study cell proliferation in the central nervous system.PMID:31869110 PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.|In Vivo:|The number of 5-Ethynyl-2′-deoxyuridine (EdU; a single injection of EdU intraperitoneally at a dose of 10, 20, 50, 100 or 200 mg/kg body weight) positive cells in the dentate gyrus slightly increased in a dose-dependent manner in two month old female mice.|References:|Chenbo Zeng, et al. Evaluation of 5-ethynyl-2′-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system. Brain Res. 2010 Mar 10;1319:21-32.An S, et al. Small-molecule PROTACs: An emerging and promising approach for the development of targeted therapy drugs. EBioMedicine. 2018 Oct;36:553-562Products are for research use only. Not for human use.|