CNBr-activated Sepharose beads (30 ml) that contains nine mg of covalently attached energetic phosphorylated (without or with one mM ATP) or inactive ERK2 were incubated with three mg of indicated purified arrestins in 60 ml of binding buffer
To conclusively dissect arrestin-dependent and arrestin-independent mechanisms, we compared WT MEFs, exactly where ERK1/2 can be activated through each pathways, and DKO MEFs lacking nonvisual arrestins , in which onlyMEDChem…