Ic acids and their esters), terpenoids, steroids, sugars and amino acids have already been detected in raw propolis, but its composition is qualitatively and quantitatively variable, based around the vegetation at the internet site from which it was collected and also the collection season (Koo et al., 1999; Bankova, 2005; Tosi et al., 2007; Valencia et al., 2012). The principle constituents of propolis in Europe, China, and North America are flavonoids and phenolic acid esters (Bankova et al., 2000; Chen et al., 2004; Lotti et al., 2010). Brazilian propolis contains, principally, diterpenes, lignans, p-coumaric acid derivatives, sesquiterpenes and acetophenones (Bankova, 2005; Piccinelli et al., 2005). Propolis can be a non-toxic organic substance with several pharmacological properties which includes cytostatic, hepato-protective, antioxidant and anti-inflammatory (Burdock, 1998; Kujumgiev et al.Voclosporin , 1999; Borrelli et al., 2002; Russo et al., 2002). It truly is also regarded as as an option in the treatment and prevention of a lot of infectious illnesses, since it displays a wide array of antimicrobial activity against a variety of bacteria, fungi, parasites and virus (Bankova et al., 1995; Kujumgiev et al., 1999; Sforcin et al., 2000; Orsi et al., 2005). As a result of this wide selection of biological activities, propolis is made use of in food industry, cosmetology, and complementary medicine products. These observations emphasize the will need to extend our knowledge about the chemical and biological characterization of propolis, which would aid together with the suitable use of this natural product in human health (Valencia et al., 2012). Dental caries are identified to be one of essentially the most prevalent and pricey oral infectious diseases worldwide (Dye et al., 2007); it is actually a multifactorial infectious disease in which diet regime, nutrition, microbial infection, and host response all play crucial roles. Dental plaque is often a common bacterial biofilm that includes mutans streptococci and other oral bacteria and their merchandise. Amongst them, Streptococcus mutans and S. sobrinus have already been recognized to become the mayor causative agent of dental caries in humans (Loesche, 1986; Smith, 2002). Some naturals compounds based in tea (HamiltonMiller, 2001), cranberries (Steinberg et al.Mirvetuximab , 2004), cacao (Osawa et al.PMID:23319057 , 2001), herbal extracts (Limsong et al., 2004) and propolis (Bankova et al., 1995; Koo et al., 2000, 2002a, 2002b; Duarte et al., 2006;), have shown inhibition of biofilm and caries improvement in some species of mutans streptococci, getting propolis and its polyphenolic compounds, by far the most studied. The biofilm formation is very important for the progression of dental caries and hence, inhibition of this aspect is among the methods at present made use of to stop this disease (Xiao et al., 2007). In view of the foregoing considerations the aim with the present study was to figure out each the chemical and botanical characterization and to decide the biological activity on mutans streptococci of 20 propolis samples obtained in central and southern Chile.Components and MethodsPreparation of ethanolic and methanolic extracts of propolis (EEP) Twenty propolis samples were obtained from a variety of beekeeping producers in the central and southern area (Valpara o, Metropolitana, Libertador Bernardo O’Higgins and La Araucan Regions), Chile. Concentrated ethanol and methanol extracts (EEP) have been ready with 30 g of chopped fresh propolis, which have been macerated for 7 days at area temperature, covered with ethanol (70 ) in volumetric flask (100 mL).