S for the human CD141+ dermal DC subset, the porcine CD163lowCD172a+ subset towards the human CD1c+ dermal DC, plus the porcine CD163+CD172a+ subset to the monocytederived human CD14+ dermal DC subset. In porcine tonsils, plasmacytoid DC (CD172alowCD4+MHCIIlow) and conventional DC (cDC1, CD172a-/lowCADM1++MHCII++ and cDC2, CD172a++CD14-CD163-MHCII ++CADM1low) but additionally monocyte-derived cells (CD172a++CD163-CD14+MHCII++) and macrophage-like cells (CD172a++, CD163+CD14-MHCII++) have already been identified [1751]. Compared to blood, not all tonsil-resident cDC1 and cDC2 cells express the C-type lectin receptor DEC205 [1773]. All reagents and Abs for porcine myeloid cell staining shown in Figs. 200 and 201 are listed in Table 81. For analyzing neutrophils, eosinophils and basophils in porcine complete blood, granulocytes are identified primarily based on their high SSC and FSC Axl Proteins supplier properties and expression of CD172a. To separate among them, SWC8, a marker defined by the International Workshop on Swine Leukocyte Differentiation Antigen (A. [1774]), SWC1 (now identified becoming porcine CD52 [1775]) and the antigens 2B2 and 6D10 [1776] is often used. Mature neutrophils in blood express CD172a+SWC8++CD52+6D10+2B2+CD14+, whereas mature eosinophils are CD172a+SWC8++CD52-6D10-2B2+CD14- and mature basophils will recognize as CD172a +SWC8- CD52+6D10-2B2+CD14- [1762].Author Manuscript Author Manuscript Author Manuscript Author