Images were taken from the same part of the ischemic hippocampus
ated genome from nuclease degradation. In the HIV-1 RNA genome, a G-4 structure has been proposed to promote dimerization of the two viral genome copies. We present here the first…
It is used here as a marker for this axonal subset
acturer's instructions. Cells were then washed and centrifuged on a 062% discontinuous percoll gradient for 30 min at 1000 g. The neutrophil-enriched pellet was harvested, and the cells were washed…
Echocardiography was performed on lightly anesthetized mice, as described previously
s. A probability value of less than 0.05 was considered statistically significant. 5 Adrenomedullin in CNV Results To elucidate the involvement of ADM signaling in CNV, we first investigated the…
Significant lung metastasis was observed in control B16F10 but not in clone 2 cells injected mice
its activity. In contrast, acetone preserved MPO activity, and over three different concentrations of MPO, we were able to recover 9665.2% of activity. This validated acetone protein precipitation as a…
For expression, the plasmids were transformed into Escherichia coli BL21 cells
and cell growth. Additionally, eIF3a functions as a translational regulator for several key nucleotide excision repair proteins. Taken together, these properties of eIF3a may explain observations that eIF3a over-expression occurs…
As seen in Fig. 5E, a significant suppression of aB crystallin was achieved by siRNA silencing
o elucidate the relative insensitivity of rKir1.1 channel towards animal toxins. First, rKir1.1 channel had a unique turret structure which formed a narrow pore entryway for animal toxins. Different types…
As seen in Fig. 5E, a significant suppression of aB crystallin was achieved by siRNA silencing
o elucidate the relative insensitivity of rKir1.1 channel towards animal toxins. First, rKir1.1 channel had a unique turret structure which formed a narrow pore entryway for animal toxins. Different types…
Negative controls were obtained by replacement of primary antiserum with buffer
iparum was used for apicoplast immunofluorescence. The immunofluorescence protocol was similar to that reported with a few minor changes. A solution of 4% paraformaldehyde and 0.1% Triton X-100 was used…
We first optimized the conditions for cell transfection and tested MKP-3 expression
ssary to reliably define hit compounds in the context of a highthroughput screen using the assay conditions reported in this study. Notably, TFEB overexpression resulted in a 3-fold increase in…
The LNA probes were a gift from Dr Thomas Tuschl’s lab
iate Rag2 dependent recombination and Rag2 expression is highest in small resting DPs as seen in pre-T LBL. Indeed, Fli-1 binding sites have been found in the Notch1 promoter in…