The plasmid was linearized with HindIII and used as a template for SP6 RNA polymerase to generate the antisense probe or was linearized with BstxI and transcribed with T7 RNA polymerase to generate the sense probe
The plasmid was linearized with HindIII and utilised as a template for SP6 RNA polymerase to generate the antisense probe or was linearized with BstxI and transcribed with T7 RNA…