Name :
Apolipoprotein A-II/APOA2 Protein
Description :
APOA2 is a protein implicated in triglyceride, fatty acid and glucose metabolism. In pigs, the APOA2 gene is located on pig chromosome 4 (SSC4) in a QTL region affecting fatty acid composition, fatness and growth traits.
Species :
Mouse
Uniprotkb :
HEK293
Tag :
C-hFc
Synonyms :
APOA2, Apolipoprotein A-II(1-76), Apolipoprotein A-II, Apolipoprotein A2, Apo-AII, ProapoA-II
Construction :
Recombinant Mouse APOA2 Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Ala19-Lys102.[Accession |P09813]
Protein Purity :
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight :
The protein has a predicted MW of 36.2 kDa. Due to glycosylation, the protein migrates to 40-45 kDa based on Tris-Bis PAGE result.
Endotoxin :
Less than 1EU per μg by the LAL method.
Formulatione :
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution :
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage :
-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping :
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background :
APOA2 is a protein implicated in triglyceride, fatty acid and glucose metabolism. In pigs, the APOA2 gene is located on pig chromosome 4 (SSC4) in a QTL region affecting fatty acid composition, fatness and growth traits.
References and Literature :
1. Ballester M, et al. Analysis of the porcine APOA2 gene expression in liver, polymorphism identification and association with fatty acid composition traits. Anim Genet. 2016 Oct;47(5):552-9. doi: 10.1111/age.12462. Epub 2016 Jun 14. PMID: 27296287.
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