Name :
MTHFS Protein

Description :
5-formyltetrahydrofolate cyclo-ligase (MTHFS) belongs to the 5-formyltetrahydrofolate cyclo-ligase family. It is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, contributes to tetrahydrofolate metabolism. MTHFS helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion.

Species :
Human

Uniprotkb :
E. coli

Tag :
C-6His

Synonyms :
5-formyltetrahydrofolate cyclo-ligase, 10-methenyl-tetrahydrofolate synthetase, MTHFS, 5, Methenyl-THF synthetase

Construction :
Recombinant Human 5-formyltetrahydrofolate Cyclo-ligase is produced by our E.coli expression system and the target gene encoding Met1-Ala203 is expressed with a 6His tag at the C-terminus.

Protein Purity :
Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Molecular Weight :
28 KDa, reducing conditions

Endotoxin :
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

Formulatione :
Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 200mM Nacl, 1mM DTT, 50% Glycerol, pH 8.0.

Reconstitution :

Stability & Storage :
Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Shipping :
The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below.

Research Background :
5-formyltetrahydrofolate cyclo-ligase (MTHFS) belongs to the 5-formyltetrahydrofolate cyclo-ligase family. It is an enzyme that catalyzes the conversion of 5-formyltetrahydrofolate to 5,10-methenyltetrahydrofolate, contributes to tetrahydrofolate metabolism. MTHFS helps regulate carbon flow through the folate-dependent one-carbon metabolic network that supplies carbon for the biosynthesis of purines, thymidine and amino acids. An increased activity of the encoded protein can result in an increased folate turnover rate and folate depletion.

References and Literature :

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