Name :
Human LAG-3 / CD223 Protein, His Tag (MALS verified)
Background :
Lymphocyte activation gene 3 protein (LAG3) is also known as CD antigen CD223 and protein FDC, which belongs to immunoglobulin (Ig) superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. LAG3 /CD223 involved in lymphocyte activation. LAG3 /CD223 binds to HLA class-II antigens.
Biological Activity :
Immobilized Human LAG-3, His Tag (Cat. No. LA3-H5222) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human LAG3 Antibody, Human IgG1 with a linear range of 0.2-4 ng/mL (QC tested).
Species :
Source :
Human LAG-3, His Tag (LA3-H5222) is expressed from human 293 cells (HEK293). It contains AA Leu 23 – Leu 450 (Accession # P18627-1 ).
Tag :
Synonyms :
（Synonym）LAG3,CD223,FDC
Purity :
（Purity）>95% as determined by SDS-PAGE.
Storage and Stability :
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin Level :
（Endotoxin）Less than 1.0 EU per μg by the LAL method.
Formulation :
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Protein Structure :
This protein carries a polyhistidine tag at the C-terminus, and has a calculated MW of 47.1 kDa. The predicted N-terminus is Leu 23. The reducing (R) protein migrates as 55-60 kDa in SDS-PAGE due to glycosylation.
Refactoring Approach :
Please see Certificate of Analysis for specific instructions.
Protein Labeling :
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