Product Name :
Anti-His tag, AlpSdAbs® VHH(Biotin)
Applications:
ELISA,Purification,BLI,SPR
Reactivity :
His tag
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-His tag, AlpSdAbs® VHH(Biotin) is designed for detecting His tag fusion proteins.{{2924858-25-1} medchemexpress|{2924858-25-1} Technical Information|{2924858-25-1} Data Sheet|{2924858-25-1} manufacturer} Anti-His tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to His tag coupled to Biotin, and Anti-His tag, AlpSdAbs® VHH(Biotin) detects the His tag selectively, no reactivity with other proteins. | Immunogen: 6*His tag fusion protein | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: Biotin | Specificity: His tag sequence(HHHHHH) | Cross-Reactivity: Highly selective for His tag sequence | Affinity: Dissociation constant KD of 0.1nM | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Description2 :
Anti-His tag, AlpSdAbs® VHH(Biotin) is designed for detecting His tag fusion proteins. Anti-His tag, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to His tag coupled to Biotin, and Anti-His tag, AlpSdAbs® VHH(Biotin) detects the His tag selectively, no reactivity with other proteins.
Immunogen:
6*His tag fusion protein
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
10mM PBS (pH 7.{{347174-05-4} site|{347174-05-4} Protocol|{347174-05-4} Description|{347174-05-4} supplier} 5), 0.PMID:30969670 05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage :
Function:
WB: 1:5,000-1:20000 ELISA: 1:5,000-1:20000IP: 1-2ug/sampleBLI (biolayer interferometry)SPR (surface plasmon resonance)Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.